Column Descriptions and Usage

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CSC Columns

The core of any chromatographic technique comes down to the column. GC, HPLC, SFC all require specific columns. Each column has its own use case, and will be good for different types of analytes. While this guide doesn’t go through the specific techniques, and shouldn’t be taken as universal, it is hopefully a starting point if you don’t know what columns the CSC has and what they are useful for. We have 3 main types of columns: reverse phase LC, normal phase LC/SFC, and GC.

Reverse Phase LC Columns

  • Acquity BEH C18, 2.1x50 mm, 1.7 um - General nonpolar molecule separation
  • Acquity BEH C18, 2.1x100 mm, 1.7 um - Difficult nonpolar molecule separation
  • Acquity BEH C8, 2.1x100 mm, 1.7 um - General nonpolar molecule separation
  • InfinityLab C18, 4.6x250 mm, 5 um - Method scouting for prep scale purification, small scale purification (<10 mg)
  • Pursuit XRS C18, 21.2x250 mm, 5 um (matched to InfinityLab C18) - Prep scale purification

SFC Columns

  • Torus BEH, 2.1x50 mm, 1.7 um - Polar achiral separations
  • Torus DIOL, 2.1x250 mm, 5 um - Organic separations with hydrogen bonding substrates
  • Torus 1-AA, 2.1x250 mm, 5 um - Organic separations with aromatic substrates
  • Torus 2-PIC, 2.1x250 mm, 5 um - Organic separations with aromatic or hydrogen bonding substrates
  • Torus DEA, 2.1x250 mm, 5 um - Organic separations with hydrogen bond donors
  • Daicel IA, 4.6x150 mm, 3 um - Chiral separations
  • Daicel IBN, 4.6x150 mm, 3 um - Chiral separations
  • Daicel IC, 4.6x150 mm, 3 um - Chiral separations
  • Daicel IG, 4.6x150 mm, 3 um - Chiral separations
  • Viridis AMY1 (similar to Daicel AD), 4.6x150 mm, 3 um - Chiral separations
  • Viridis CEL1 (similar to Daicel OD), 4.6x150 mm, 3 um - Chiral separations
  • Viridis CEL2 (similar to Daicel AZ), 4.6x150 mm, 3 um - Chiral separations

GC Columns

  • TG-5MS (5% phenyl/95% dimethylpolysiloxane) - Active organic compounds including acids and bases
  • 5 A PLOT (5 Å mol sieves) - Permanent gases (H2, CO2, CO, etc.)
  • DB-WAX (polyethylene glycol) - Polar compounds including fatty acids, alcohols, and aldehydes
  • DB-1701 (14% cyanopropylphenyl/86%methylpolysiloxane) - Mid-polarity including alcohols, sugar derivatives, etc.
  • AT-Aquawax-DA  (PEG-nitroterephthal acid) - Aqueous injections of small molecule acids
  • TB-Bond Q (100% divinylbenzene) - Permanent gases (CO2, CH4, O2, N2, CO)
  • GS-Carbonplot (carbon layer) - inorganic and organic gases
  • DB-1 (100% dimethylpolysiloxane) - high temperature, wide variety of nonpolar compounds
  • Chiraldex 73033 (2,6-di-o-pentyl-3-trifluoroacetyl γ-cyclodextrin) - Chiral separations